Virtual Labs

Genetic transformation techniques- Gene gun mediated, Agrobacterium mediated transformation - Overexpression, antisense expression (in model as well as crop plants)

1.What is the main role of Agrobacterium in plant genetic transformation?

a: To act as a nutrient source for plants Explanation

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b: To naturally insert T-DNA into plant cells Explanation

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c: To create resistant genes within plants Explanation

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d: To enhance photosynthesis in plants Explanation

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2.Which material is commonly used for microcarrier particles in particle bombardment?

a: Silver Explanation

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b: Iron Explanation

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c: Gold or tungsten Explanation

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d: Copper Explanation

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3.What does acetosyringone do in Agrobacterium-mediated transformation?

a: Inhibits bacterial growthwer1 Explanation

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b: Activates virulence genes Explanation

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c: Destroys plant cell walls Explanation

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d: Acts as a nutrient source for the plant Explanation

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4.What is the primary advantage of particle bombardment over Agrobacterium-mediated transformation?

a: It is less expensive Explanation

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b: It can be used for a wider range of plant species Explanation

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c: It uses fewer chemicals Explanation

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d: It requires less preparation time Explanation

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5.What stops the macrocarrier before particles reach the target cells in particle bombardment?

a: A pressure valve Explanation

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b: The target cell plate Explanation

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c: A stopping screen Explanation

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d: A helium burst Explanation

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6.Which Agrobacterium strain is commonly used for genetic transformation?

a: A. tumefaciens Explanation

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b: A. rhizogenes Explanation

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c: E. coli Explanation

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d: Bacillus subtilis Explanation

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7.In Agrobacterium-mediated transformation, why are plant cells co-cultivated in the dark?

a: To prevent chlorophyll synthesis Explanation

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b: To minimize cell damage from light exposure Explanation

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c: To improve transformation efficiency by affecting hormone levels Explanation

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d: To reduce bacterial growth Explanation

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8.How is the genetic material delivered into plant cells during particle bombardment?

a: Using a needle injection Explanation

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b: Via a vacuum infiltration process Explanation

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c: By a high-pressure blast of helium gas Explanation

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d: Through bacterial transformation Explanation

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