Aim of the experiment

The aim of this experiment is to understand, apply, and analyse the principles of gene isolation, cloning, and characterization in plants through the use of recombinant DNA technology. This includes the identification and isolation of a gene of interest, its insertion into a suitable cloning vector, and the introduction of the recombinant DNA molecule into a host cell for replication and study.

The experiment also aims to explain the structural organization of genes such as promoter regions, coding sequences, and untranslated regions and to analyse their roles in gene expression and regulation. By studying these processes, learners will be able to apply molecular techniques involved in recombinant DNA formation and evaluate the significance of gene cloning in understanding gene function and in improving crop traits such as pest resistance, stress tolerance, and enhanced nutritional quality.