Computation of various physical and chemical properties of Proteins and DNA from their primary sequence
1. Which of the following amino acids is most likely to be found in the core of the protein structure?
2. Which of the following techniques can be used to identify a post-translational modification such as phosphorylation or methylation of a protein, and the residue where it is modified?
3. Which amino acids do you expect to find in higher proportion within histones compared to a cytoskeletal protein?
4. Which of the following has the highest isoelectric point?
5. Two solutions of the same protein, with concentrations of 10-3 and 5X10-4 M, are kept in two different cuvettes with path lengths 1 cm and 2 cm, respectively. If the absorbance for 1st sample is 1.2,what will be the absorbance for the 2nd sample?
6. Which one of the following changes will not alter the amino acid sequence of the encoded protein?
7. Two samples of DNA, A and B have melting temperatures of 380K and 320K respectively. This is because
8. What is the starting point for the selection of a suitable Ion exchange matrix for the purification of a recombinant protein?
9. Protein ‘A’ contains 192 amino acids and is mono-ubiquinated at two residues. What could be the approximate molecular weight of the protein? [Molecular weight of ubiquitin is 8 kDa]
10. If we have a polypeptide A of size 11kDa, what would be the mass of the open reading frame that codes for the polypeptide? The average mass of an amino acid and a nucleotide is considered to be 110Da and 330 Da, respectively.