Tools

Metabolic flux determination, pathway engineering in plants

Further reading:

  1. Calvin, M. (1961). The Path of Carbon in Photosynthesis. Nobel Lecture. This lecture provides a comprehensive overview of the experiments using carbon-14 (^14C) to trace the incorporation of CO₂ into organic compounds in Chlorella pyrenoidosa, leading to the discovery of the Calvin cycle.
  2. Benson, A. A. (2002). Following the path of carbon in photosynthesis: a personal story. Photosynthesis Research, 73(1-3), 29–49. An autobiographical account detailing the collaborative efforts and methodologies employed in the discovery of the carbon fixation pathway.
  3. Kok, B., Forbush, B., & McGloin, M. (1970). Cooperation of charges in photosynthetic O₂ evolution—I. A linear four step mechanism. Photochemistry and Photobiology, 11(6), 457–475. Introduced the Kok cycle, describing the stepwise accumulation of oxidizing equivalents in PSII leading to water oxidation and O₂ evolution.
  4. Nickelsen, K. (2007). Explaining Photosynthesis: Models of Biochemical Mechanisms, 1840–1960. Springer. Offers a historical analysis of the development of photosynthesis research, including the role of radioisotopes.

References

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  2. Drouin, G., Godin, J.R., and Pagé, B. (2011). The genetics of vitamin C loss in vertebrates. Current Genomics 12: 371–378. https://doi.org/10.2174/138920211796429736
  3. Harrison, E.H. (2005). Mechanisms of digestion and absorption of dietary vitamin a. Annual Review of Nutrition 25: 87–103. https://doi.org/10.1146/annurev.nutr.25.050304.092614
  4. Trumbo, P., Yates, A.A., Schlicker, S., and Poos, M. (2001). Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. Journal of the Academy of Nutrition and Dietetics 101: 294
  5. Ye, X., Al-Babili, S., Klöti, A. et al. (2000). Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287: 303–305. https://doi.org/10.1126/science.287.5451.303
  6. Paine, J.A., Shipton, C.A., Chaggar, S. et al. (2005). Improving the nutritional value of Golden Rice through increased pro-vitamin a content. Nature Biotechnology 23: 482. https://doi.org/10.1038/nbt1082
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  13. Diretto, G., Welsch, R., Tavazza, R. et al. (2007). Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers. BMC Plant Biology 7: 11. https://doi.org/10.1186/1471-2229-7-11
  14. Van Eck, J., Conlin, B., Garvin, D.F. et al. (2007). Enhancing beta-carotene content in potato by RNAi-mediated silencing of the beta-carotene hydroxylase gene. American Journal of Potato Research 84: 331. https://doi.org/10.1007/BF02986245
  15. Zeng, J., Wang, X., Miao, Y. et al. (2015). Metabolic engineering of wheat provitamin A by simultaneously overexpressing CrtB and silencing carotenoid hydroxylase (TaHYD). Journal of Agricultural and Food Chemistry 63: 9083–9092. https://doi.org/10.1021/acs.jafc.5b04279
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