Metabolic flux determination, pathway engineering in plants
1. Metabolic flux refers to:
2. What does isotopic labeling help with in metabolic studies?
3. In a metabolic network, nodes usually represent:
4. CRISPR/Cas9 is used in plant pathway engineering to:
5. Metabolic engineering often targets which part of the plant cell?
6. A pathway shows high metabolite accumulation but low flux. What is the most likely explanation?
7. Why does overexpression of phytoene synthase alone often fail to strongly increase carotenoid content?
8. Which parameter cannot be directly inferred from steady-state MFA?
9. Which strategy best improves carotenoid accumulation without increasing metabolic burden?
10. In flux balance analysis (FBA), changing the objective function from “biomass” to “product synthesis” will: