Step 1: Switch on the TEM machine
Step 2: Remove the sample holder, so a required sample can be inserted for observation.
Step 3: Select any of CrCoNi alloy, Monocrystalline Ag or Vanadium carbide sample. Grab the sample and place it on front end of TEM holder.
Step 4: Click ‘Insert’ for placing the sample for observation in TEM.
Step 5: Pull the vacuum to ‘HV’ and click ‘Set’
Step 6: Pull the Accelerating voltage to 200 kV and click ‘Set’.
Step 7: Now, click ‘BEAM ON’, which will make the system ready for observations. A corresponding image will be observed on the image panel.
Step 8: ‘Bright field mode’ or ‘dark field mode’ can be selected’ for observation.
Step 9: Choose appropriate magnification for observing the dislocation partials.
Step 10. Find the distance between the dislocation partials for estimating the stacking fault energy.
Step 11: Repeat the process for estimating stacking fault energy of other samples as well.