Proteins analysis using polyacrylamide gel electrophoresis (PAGE)
1. Electrophoresis is defined as the migration of _____to the ____charged electrode under the influence of an electric field
2. The electrophoretic mobility (μ) is the velocity of a macromolecule under a constant electric field. μ of a biomolecule is influenced by its
3. SDS-PAGE is an electrophoretic technique commonly used for analysing ________.
4. In Polyacrylamide Gel Electrophoresis, proteins will migrate towards the ________.
5. In SDS-PAGE, the protein molecules are separated on the basis of
6. To study the constituents of protein complex, which type of gel electrophoresis is not preferred?
7. In SDS-PAGE, _________ renders a uniform negative charge to the proteins.
8. Electrophoretic velocity (v) of the protein samples _________ with the increase in voltage.
9. In reducing SDS-PAGE, _________ is used to break disulphide linkages in proteins.
10. To visualize the protein bands after separation using SDS-PAGE, which of the stains is used?