Proteins analysis using polyacrylamide gel electrophoresis (PAGE)
1. In PAGE, acrylamide and ________ polymerize to form the gel in SDS-PAGE.
2. In the gel polymerization reaction, ammonium persulfate (APS) and TEMED act as
3. The pore size of the gel in PAGE is the key player in the molecular sieving and separation of proteins. For studying proteins of small molecular weight, the gel must have
4. In Laemmli buffer of SDS-PAGE, ________ is used as loading dye.
5. Glycerol is added to the protein samples before loading them into the wells of the gel. This is because
6. The pH of (i) stacking gel, (ii) resolving gel and (iii) tank buffer in SDS PAGE is _________respectively.
7. An SDS molecule binds at an interval of every _________ amino acids.
8. For high-resolution study of protein separated by SDS-PAGE, which staining technique may be used?
9. If a protein sample shows a molecular weight of 100kDa in when analysed by PAGE and gives a band at 20kDa and another at 30kDa molecular weight in SDS-PAGE, what is the stoichiometry of the protein complex?
10. In BN-PAGE, the electrophoretic separation of the protein samples is based on _________.