Kinetic characterization of protein-ligand binding by surface plasmon resonance (SPR)
1. Surface plasmon resonance is used to study which type of molecular interactions?
2. Which of the following materials is commonly used to coat the SPR sensor chip?
3. The detection unit of the SPR sensors detects which of the following parameters?
4. When there is an increase in the refractive index of the medium in contact to the metal surface, the SPR angle
5. The primary advantage of using SPR for protein-protein interaction is
6. The RU in the SPR sensorgram stands for
7. Which phase of the SPR experiment depicts the injection of an interacting molecule (analyte) over the immobilized ligand?
8. The binding kinetics between two proteins depends on which of the following parameters?
9. What is the relation between the dissociation constant (KD) and binding affinity between two interacting proteins?
10. Covalent immobilization of ligand on the sensor chip is achieved using