Protein purification by affinity chromatography

Objective

Affinity chromatography is a biophysical technique that exploits the unique biological function or the chemical structure of a biomolecule such that it specifically binds to an immobilized ligand reversibly and can be separated from a mixture of several biomolecules. This chromatographic technique helps to achieve the following.

  1. High specificity of purification: Selectively purify the target protein with high specificity while minimizing contamination from other proteins.
  2. High purity: Obtain a highly pure target protein.
  3. Retention of Bioactivity: Maintain the structural and functional integrity of the protein.
  4. Efficient Yield: Maximize the recovery of the target protein.
  5. Rapid Purification: Achieve fast and efficient separation with fewer purification steps.