Virtual Labs

Virtual experiments to study characteristics of plant cells and genomes

1. What is the first step in preparing a transverse section of a leaf for microscopic observation?

a: Staining the leaf with iodine Explanation

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b: Embedding the leaf in wax Explanation

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c: Cutting a thin slice using a new razor blade Explanation

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d: Hydrating the leaf in water Explanation

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2. In the procedure of a stem transverse section, which stain is used to distinguish between xylem and phloem?

a: Iodine Explanation

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b: Toluidine blue Explanation

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c: Safranin Explanation

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d: Acetocarmine Explanation

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3. What is the purpose of placing the prepared stem transverse section in water during the experiment?

a: To soften the tissue for slicing Explanation

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b: To keep the section hydrated during observation Explanation

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c: To highlight vascular tissue Explanation

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d: To wash away excess stain Explanation

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4. After staining and mounting a root transverse section on a slide, what is the correct order for microscopic observation?

a: Start with the highest magnification first Explanation

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b: Start with low magnification, then gradually increase Explanation

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c: Apply heat to the slide before observation Explanation

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d: Dehydrate the section with alcohol before viewing Explanation

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5. What procedural mistake could result in poorly defined root epidermis cells in a transverse section?

a: Failure to use a microtome for sectioning Explanation

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b: Overstaining the section Explanation

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c: Insufficient dehydration before embedding Explanation

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d: Using glycerine instead of water for mounting Explanation

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