Effect of physical and chemical environment on growth
1. Which of the following is true about acidophiles?
2. What is the purpose of autoclaving the LB broth?
3. What does turbidity in the culture tubes after incubation indicate?
4. What is the optimal pH for the growth of E. coli based on the experiment?
5. You are an environmental microbiologist working on a project to remediate a contaminated mining site. The soil compaction leads to low oxygen availability. Which type of microorganisms would you select for remediation under these conditions?
6. What is the observed result for E. coli growth at pH 7 and 37°C?
7. Why is it important to use sterile equipment and maintain aseptic techniques during this experiment?
8. Why is the wavelength of 600 nm used to measure the absorbance of the E. coli culture?
9. Why is sodium chloride added to the LB broth?
10. Which phase of bacterial growth is characterized by the highest rate of cell division?