Virtual Labs

Tools

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

An introduction to Next Generation Sequencing (NGS)

Choose difficulty:

Beginner

Intermediate

Advanced

Determining the order of A, T, G ,C in a strand of DNA

a: Ligation Explanation

Explanation

b: DNA sequencing Explanation

Explanation

c: PCR Explanation

Explanation

d: Recombination Explanation

Explanation

Chain termination in sanger sequencing is due to the presence of

a: Primer Explanation

Explanation

b: Nucleotides Explanation

Explanation

c: ddNTP Explanation

Explanation

d: dNTP Explanation

Explanation

Typical workflow in NGS

a: Sample Preparation, DNA Sequencing, Data Analysis, Mutation calling Explanation

Explanation

b: Library Preparation, Sample analysis, DNA sequencing, Data analysis Explanation

Explanation

c: Sample preparation, Creating Library, DNA sequencing, Data Analysis Explanation

Explanation

d: Constructing Library, Fragmenting DNA, Data Analysis, PCR amplification Explanation

Explanation

Bioinformatics tools are import in NGS as it includes

a: Removing of low-quality sequence reads Explanation

Explanation

b: Sequence alignment Explanation

Explanation

c: Identifying mutation Explanation

Explanation

d: All the above Explanation

Explanation

Community Links Sakshat Portal Outreach Portal FAQ: Virtual Labs

AGPL 3.0 & Creative Commons (CC BY-NC-SA 4.0)