Virtual Labs

Cloning and expression of GFP sequence/gene in the expression vector

1. What does GFP stand for?

a: Green Fluorescent Protein Explanation

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b: Gene Fragment Primer Explanation

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c: General Fluorescence Protocol Explanation

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d: Genomic Fusion Product Explanation

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2. What is an expression vector?

a: A vector used for cloning and amplification of DNA sequences. Explanation

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b: A vector used for transferring proteins into bacterial cells. Explanation

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c: A vector used for labeling cells with fluorescent dyes. Explanation

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d: A vector used for studying gene regulation in eukaryotic cells. Explanation

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3. Which organism naturally produces GFP?

a: Bacteria Explanation

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b: Fungi Explanation

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c: Jellyfish Explanation

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d: Plants Explanation

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4. Which color does GFP emit when exposed to UV or blue light?

a: Red Explanation

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b: Green Explanation

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c: Blue Explanation

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d: Yellow Explanation

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5. Which technique is commonly used to visualize GFP expression in cells or tissues?

a: Western blotting Explanation

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b: PCR amplification Explanation

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c: Fluorescence microscopy Explanation

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d: Gel electrophoresis Explanation

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6. What is the role of a selectable marker in an expression vector?

a: To facilitate the binding of RNA polymerase Explanation

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b: To enable the identification of cells that have taken up the vector Explanation

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c: To enhance the replication of the vector Explanation

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d: To fluoresce under UV light Explanation

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7. Which of the following is the primary purpose of cloning the GFP gene into an expression vector?

a: To study the function of an unknown protein Explanation

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b: To produce a fluorescent marker for monitoring gene expression Explanation

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c: To enhance the growth rate of bacteria Explanation

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d: To inhibit the growth of pathogens Explanation

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8. Which of the following is NOT a component of a typical expression vector used for GFP expression?

a: Origin of replication Explanation

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b: Promoter Explanation

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c: Antibiotic resistance gene Explanation

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d: DNA ligase gene Explanation

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9. Why is it important to include a terminator sequence in an expression vector containing the GFP gene?

a: To start the transcription process Explanation

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b: To stop the transcription process and ensure proper mRNA termination Explanation

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c: To enhance translation efficiency Explanation

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d: To bind transcription factors Explanation

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10. What is the advantage of using GFP as a reporter gene in molecular biology experiments?

a: GFP is a small protein Explanation

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b: GFP fluoresces without requiring additional substrates or cofactors Explanation

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c: GFP can bind to DNA Explanation

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d: GFP enhances cell growth Explanation

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