Cloning and expression of GFP sequence/gene in the expression vector
1. Which method is commonly used to confirm the successful cloning of the GFP gene into the expression vector?
2. Which method is commonly used for the amplification of the GFP gene during cloning?
3. What is the purpose of a selectable marker in an expression vector used for GFP cloning?
4. Which class of enzymes is commonly used for DNA ligation during GFP gene cloning?
5. Which region of the GFP gene is responsible for the protein's fluorescent properties?
6. What is the primary purpose of using an expression vector in cloning the GFP gene?
7. Which method is used to monitor the cutting and joining reactions of the vector and GFP gene?
8. What is the function of the ligation reaction in the cloning process?
9. What is the purpose of an origin of replication (ori) in an expression vector?
10. Which of the following is a common method used to introduce recombinant DNA into E. coli cells?