Virtual Labs

Flow Cytometry of the Immune Cells

1. What is the purpose of flow cytometry?

a: To analyze the physical and chemical properties of cells and particles in a fluid sample Explanation

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b: To sequence DNA and RNA Explanation

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c: To visualize cells and tissues at the microscopic level Explanation

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d: To measure the mass of cells and particles in a sample Explanation

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2. What is the purpose of sheath fluid?

a: To lubricate the cells Explanation

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b: To position the sample of cells into a single file for optical interrogation Explanation

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c: To increase the speed of the flow Explanation

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d: To increase volume of the cellular mixture Explanation

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3. Which of the following is not a component of a flow cytometer?

a: Laser Explanation

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b: Fluidics system Explanation

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c: Microscope Explanation

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d: Detectors Explanation

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4. What is forward scatter in flow cytometry?

a: The measurement of light scattered at an angle to the laser beam Explanation

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b: The measurement of light emitted by fluorescent molecules in the sample Explanation

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c: The measurement of light scattered in the same direction as the laser beam Explanation

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d: The measurement of light transmitted through the sample Explanation

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5. Which of the following is not typically measured in flow cytometry?

a: Cell size Explanation

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b: Cell shape Explanation

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c: Cell surface markers Explanation

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d: Cell weight Explanation

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6. What is fluorescence in flow cytometry?

a: The measurement of light scattered at an angle to the laser beam Explanation

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b: The measurement of light emitted by fluorescent molecules in the sample Explanation

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c: The measurement of light scattered in the same direction as the laser beam Explanation

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d: The measurement of light transmitted through the sample Explanation

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7. Which characteristic of a cell is proportional to forward-scattered light (FSC) in flow cytometry?

a: Cell granularity Explanation

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b: Cell size Explanation

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c: Cell density Explanation

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d: Cell viability Explanation

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8. What does side-scattered light (SSC) in flow cytometry indicate about a cell?

a: Cell size Explanation

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b: Cell surface markers Explanation

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c: Cell granularity or internal complexity Explanation

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d: Cell viability Explanation

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9. Which type of light measurement is used to differentiate cell types in a heterogeneous cell population?

a: Only forward-scattered light (FSC) Explanation

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b: Only side-scattered light (SSC) Explanation

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c: Both forward-scattered light (FSC) and side-scattered light (SSC) Explanation

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d: Emitted fluorescence light Explanation

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10. Which of the following is NOT a component of a flow cytometer?

a: Fluidics system Explanation

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b: Optical system Explanation

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c: Signal detection and processing system Explanation

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d: Gas chromatography system Explanation

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11. What is the main function of the optical system in a flow cytometer?

a: To channel cells into a single file Explanation

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b: To focus the laser beam and detect scattered light Explanation

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c: To convert light signals into electronic signals Explanation

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d: To analyze the data and generate reports Explanation

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