Virtual Labs

1. What type of cells does the blue box indicate?

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a: Monocytes Explanation

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b: Lymphocytes Explanation

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c: Granulocytes Explanation

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d: Cell debris Explanation

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2. In the provided scatter plot, which cell type is represented by the cluster in green?

a: Erythrocytes Explanation

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b: Lymphocytes Explanation

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c: Monocytes Explanation

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d: Granulocytes Explanation

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3. Which of the following samples can be analyzed with flow cytometry?

a: Blood Explanation

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b: Urine Explanation

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c: Saliva Explanation

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d: All of the above Explanation

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4. Which of the following is an application of flow cytometry?

a: Visualizing the internal structure of cells Explanation

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b: Measuring the pH of a fluid sample Explanation

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c: Counting the number of bacteria in a sample Explanation

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d: Quantifying the amount of protein in a sample Explanation

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5. Which of the following statements is true about flow cytometry data analysis?

a: The fluorescence intensity of each cell is measured and recorded as a histogram Explanation

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b: The forward scatter signal is used to measure the size of each cell Explanation

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c: The side scatter signal is used to measure the granularity of each cell Explanation

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d: All of the above Explanation

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6. Which of the following methods is used to sort cells or particles in flow cytometry?

a: Gravity sedimentation Explanation

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b: Electrophoresis Explanation

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c: Electrostatic deflection Explanation

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d: Centrifugation Explanation

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7. Why is it important to obtain a single-cell suspension for flow cytometry?

a: To ensure cells are stained properly Explanation

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b: To allow cells to be analyzed individually Explanation

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c: To prevent cell lysis Explanation

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d: To enhance cell proliferation Explanation

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8. What can the data from a flow cytometer be used to determine?

a: The genetic sequence of cells Explanation

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b: The nutrient content of the cell culture Explanation

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c: The physical and biochemical properties of cells Explanation

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d: The electrical conductivity of cells Explanation

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9. Which sorting mechanism might a flow cytometer use to sort cells?

a: Electrostatic charge Explanation

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b: Thermal gradients Explanation

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c: Acoustic waves Explanation

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d: Centrifugal force Explanation

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10. Which of the following is NOT a typical application of flow cytometry?

a: Biomedical research Explanation

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b: Clinical diagnostics Explanation

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c: Drug discovery Explanation

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d: Soil analysis Explanation

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Flow Cytometry of the Immune Cells