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Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Contributors

Feedback

Quantitative estimation of proteins

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Why is the UV absorbance method suitable for protein quantitation?

a: It detects all types of biomolecules. Explanation

Explanation

b: It specifically measures the presence of aromatic amino acids. Explanation

Explanation

c: It provides a visual color change. Explanation

Explanation

d: It is not influenced by nucleic acids. Explanation

Explanation

What is the primary reason for using Coomassie Brilliant Blue G-250 in the Bradford assay?

a: It binds to nucleic acids. Explanation

Explanation

b: It changes color upon binding to proteins. Explanation

Explanation

c: It precipitates proteins. Explanation

Explanation

d: It has a high affinity for lipids. Explanation

Explanation

Which of the following is NOT a component involved in the Lowry assay?

a: Copper sulfate Explanation

Explanation

b: Sodium tartrate Explanation

Explanation

c: Folin–Ciocalteu reagent Explanation

Explanation

d: Ethidium bromide Explanation

Explanation

In the Beer-Lambert Law, what does the term ϵ (psilon) represent?

a: Path length Explanation

Explanation

b: Concentration of the analyte Explanation

Explanation

c: Absorbance Explanation

Explanation

d: Molar extinction coefficient Explanation

Explanation

When preparing Folin and Ciocalteu’s reagent for the Lowry assay, what is the recommended dilution ratio with water?

a: 1:1 Explanation

Explanation

b: 1:5 Explanation

Explanation

c: 1:10 Explanation

Explanation

d: 1:20 Explanation

Explanation

What is the first step in preparing Coomassie Brilliant Blue G-250 reagent?

a: Dissolve the dye in water. Explanation

Explanation

b: Dissolve the dye in 95% ethanol. Explanation

Explanation

c: Mix the dye with phosphoric acid. Explanation

Explanation

d: Add the dye to a buffer solution. Explanation

Explanation

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