2D Gel Electrophoresis of the Egg Proteins
1. In 2D gel electrophoresis, the second-dimension separation is performed at what angle relative to the first-dimension separation? :
2. Which of the following stains used for staining proteins in 2D gel electrophoresis is incompatible with mass spectrometry?:
3. In 2D gel electrophoresis, proteins are separated based on _____ in first dimension and based on _____ in second dimension.:
4. Which of the following reagents is commonly used for solubilizing proteins in the sample preparation step of 2D gel electrophoresis?
5. Isoelectric focusing (IEF) separates proteins based on their:
6. In the equilibration step of 2D gel electrophoresis, what purpose does the SDS buffer serve?
7. One of the disadvantages of 2D gel electrophoresis is:
8. What is the primary reason for equilibrating the IEF gel or IPG strip in SDS buffer before SDS-PAGE?
9. What is the primary limitation of 2D gel electrophoresis compared to other proteomic techniques?
10. Which of the following best describes the second dimension of 2D gel electrophoresis (SDS-PAGE)?