Virtual Labs

Western Blot Analysis of the Cell Lysate using Antibody

1. What is the primary role of blocking agents in Western blotting?

a: To enhance the binding of primary antibodies Explanation

Explanation

b: To reduce nonspecific binding of antibodies Explanation

Explanation

c: To increase the sensitivity of detection Explanation

Explanation

d: To stabilize the proteins on the membrane Explanation

Explanation

2. In Western blotting, which staining method is commonly used to visualize total protein on the membrane after transfer?

a: Coomassie Blue staining Explanation

Explanation

b: Silver staining Explanation

Explanation

c: Ponceau S staining Explanation

Explanation

d: Ethidium bromide staining Explanation

Explanation

3. What is the main difference between SDS and Native Page?

a: Proteins are denatured for SDS PAGE analysis, but not for NATIVE PAGE Explanation

Explanation

b: Proteins are detected using antibodies for SDS PAGE, an oligomers for NATIVE Page Explanation

Explanation

c: NATIVE PAGE involves stacking gel, while SDS PAGE does not Explanation

Explanation

d: SDS PAGE separates proteins based on size and charge, while NATIVE PAGE separates proteins based only on size Explanation

Explanation

4. What is the primary purpose of Western blotting?

a: To measure the concentration of DNA in a sample Explanation

Explanation

b: To detect specific proteins in a sample Explanation

Explanation

c: To determine the pH level of a solution Explanation

Explanation

d: To sequence RNA molecules Explanation

Explanation

5. Which of the following is NOT a step in the Western blotting process?

a: Gel electrophoresis Explanation

Explanation

b: Protein transfer to a membrane Explanation

Explanation

c: DNA amplification Explanation

Explanation

d: Antibody incubation Explanation

Explanation

6. What type of gel is commonly used in Western blotting for protein separation?

a: Agarose gel Explanation

Explanation

b: Polyacrylamide gel Explanation

Explanation

c: Starch gel Explanation

Explanation

d: Cellulose acetate gel Explanation

Explanation

7. Which type of gel electrophoresis is typically used to separate proteins by their size in Western blotting?

a: Native PAGE Explanation

Explanation

b: SDS-PAGE Explanation

Explanation

c: Isoelectric focusing Explanation

Explanation

d: Agarose gel electrophoresis Explanation

Explanation

8. What is the primary function of the stacking gel in SDS-PAGE?

a: To separate proteins based on their size Explanation

Explanation

b: To concentrate and align proteins before they enter the resolving gel Explanation

Explanation

c: To transfer proteins to the membrane Explanation

Explanation

d: To denature the proteins Explanation

Explanation

9. What is the typical pH of the stacking gel used in SDS-PAGE?

a: pH 6.8 Explanation

Explanation

b: pH 8.8 Explanation

Explanation

c: pH 7.4 Explanation

Explanation

d: pH 9.0 Explanation

Explanation

10. What is the typical pH of the resolving gel in SDS-PAGE?

a: pH 6.8 Explanation

Explanation

b: pH 8.8 Explanation

Explanation

c: pH 7.4 Explanation

Explanation

d: pH 9.0 Explanation

Explanation