Virtual Labs

Thermodynamic characterization of protein-ligand binding by isothermal titration calorimetry (ITC)

1. What is the full form of ITC?

a: Isothermal Titration Calorimetry Explanation

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b: Isothermal Titre Calorimetry Explanation

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c: Isothermal Titration Colour Explanation

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d: None of the above Explanation

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2. What do you mean by the term isothermal?

a: Constant pressure Explanation

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b: Constant temperature Explanation

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c: Constant volume Explanation

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d: None of the above Explanation

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3. What are the major features of ITC?

a: Reference cell Explanation

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b: Sample cell Explanation

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c: Adiabatic shield Explanation

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d: All of the above Explanation

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4. Fill in the blank, ΔG =…………………

a: ΔH - TΔS = RTlnK_D Explanation

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b: ΔH - TΔS = RTlnK_A Explanation

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c: ΔS - TΔH = RTlnK_D Explanation

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d: ΔH - TΔS = HTlnK_D Explanation

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5. What is an adiabatic process?

a: a process in which no heat is transferred into a system Explanation

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b: a process in which no heat is transferred into or out of a system Explanation

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c: a process in which no heat is transferred out of a system Explanation

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d: a process in which heat is transferred into or out of a system Explanation

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6. Fill in the blank, H =………… Here, E= internal energy, P= pressure, V=volume

a: H = E + PV Explanation

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b: H = E - PV Explanation

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c: H = E + P Explanation

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d: H = E + V Explanation

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7. What is net entropy change (ΔS) if T1 = T2, in the given formula

a: ΔS > 0, the process is spontaneous Explanation

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b: ΔS > 0, the process is in equilibrium Explanation

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c: ΔS = 0, the process is in equilibrium Explanation

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d: ΔS = 0, the process is spontaneous Explanation

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8. Binding affinity is typically measured and reported by the equilibrium dissociation constant (KD), which is used to evaluate and rank order strengths of biomolecular interactions.

a: The lower the KD value, the greater the binding affinity Explanation

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b: The lower the KD value, the lower the binding affinity Explanation

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c: The larger the KD value, the lower the binding affinity Explanation

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d: Both a and c Explanation

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9. What does ITC measure?

a: Temperature change Explanation

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b: Heat change Explanation

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c: pH change Explanation

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d: Volume change Explanation

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10. In an ITC experiment, a decrease in heat corresponds to:

a: An exothermic reaction Explanation

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b: No reaction Explanation

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c: An endothermic reaction Explanation

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d: An equilibrium reaction Explanation

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