Virtual Labs

Protein-DNA interaction analysis by electrophoretic mobility shift assay (EMSA)

1. How many types of nucleic acid polymers are present in the living systems?

a: One Explanation

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b: Two Explanation

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c: Three Explanation

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d: Four Explanation

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2. Which of the following most accurately describes the central dogma of biology?

a: DNA to protein to RNA Explanation

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b: Protein to RNA to DNA Explanation

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c: RNA to protein to DNA Explanation

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d: DNA to RNA to protein Explanation

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3. Fill in the blanks, DNA is _______stranded while RNA is _______stranded

a: Single, single Explanation

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b: Single, double Explanation

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c: Double, single Explanation

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d: Double, double Explanation

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4. DNA is the genetic material of the living systems that

a: Stores information and passes to next generation Explanation

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b: It is more stable than RNA Explanation

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c: It has deoxyribose sugar Explanation

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d: All of the above Explanation

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5. How many components are present in the basic unit of DNA?

a: One Explanation

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b: Two Explanation

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c: Three Explanation

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d: Four Explanation

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6. EMSA stands for?

a: Electrophoretic mobility shift assay Explanation

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b: Electrophoresis mobility shift assay Explanation

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c: Electrophoretic mobilization shift assay Explanation

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d: Electrophoresis mobilization shift assay Explanation

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7. EMSA technique used for the interaction of the

a: DNA with the proteins Explanation

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b: RNA with the proteins Explanation

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c: Nucleic acids with the nucleic acid binding proteins Explanation

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d: None of the above Explanation

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8. EMSA can determine the

a: Binding affinity of the nucleic acid-protein Explanation

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b: Can determine sequence specificity of the nucleic acid-protein interaction sites Explanation

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c: Help in determination of kinetic and thermodynamic parameters Explanation

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d: All of the above Explanation

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9. For performing EMSA what type of gel should be used?

a: Non-denaturing SDS-PAGE Explanation

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b: Denaturing SDS-PAGE Explanation

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c: NATIVE Explanation

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d: All of the above Explanation

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10. In an EMSA experiment free DNA is separated from the protein-DNA complexes in a native gel by which following principle?

a: Molecular weight Explanation

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b: Charge Explanation

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c: DNA digestion with DNAse Explanation

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d: Antibody immunoprecipitation Explanation

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