Tools

Fluorescence Microscope: To Image the nucleus of cells proliferation on biomaterial surface by fluorescence microscopy

  1. Clean and sterilize the specimen.
  2. Perform the cell culture or other experiment on the specimen for the desired period.
  3. Dehydrate the specimen to stop the motion of the cells in case of cell culture.
  4. Fix the specimen using glutaraldehyde.
  5. Wash in PBS.
  6. Treat it with Triton and then again wash in PBS.
  7. Treat the specimen with the Hoechst dye.
  8. Place the suitable filter in the optical axis and turn on the mercury vapor lamp.
  9. Adjust the intensity of the excitation light.
  10. Keep the specimen under the fluorescence microscope.
  11. Focus the desired region using eyepiece to view the nucleus of the specimen.
  12. Capture the digital images using digital camera for the Hoechst dye.