Determination of Fluorescence Quantum Yield of a Fluorophore
Pre-Assignment
- If the intensities of the sample and reference beam are both the same, what is the value of absorbance?
- What is the % transmission for an absorbance of 1?
- What is the unit of the absorbance?
- 'HOMO-LUMO electronic transitions are properties of the groups of atoms.' Is the statement true or false?
- What kinds of electronic transitions are observed in carbonyl compounds?
- How are the absorbance and transmittance related at the spectral peak position?
Post-Assignmnt
- What kind of cuvettes (glass or quartz) will you use for a wavelength scan spanning the UV-visible range?
- What do you expect if you run the wavelength scan by mistakenly placing the sample in the reference holder and the sample blank or reference in the sample holder?
- It is necessary not to touch the cuvette on sides that come across the light path and wipe out gently all the sides with lint free paper. Why?
- What does an absorption spectrum of a sample tell about the colour of the sample?
- Though caffeine absorbs light, yet it is colourless. Why?
- How do the samples look like in following two cases: when absorbance values of the samples are (i) close to zero and (ii) very high in the entire visible range?
- Which compound of the two studied here absorbs light of higher energy per photon?
- Which colors of light do caffeine and potassium dichromate absorb?