This calculator uses a standard curve plot (forced through origin) to calculate the concentration of unknown samples. Check sample data table (Open Table)

Next Step

Enter absorbance (540 nm) and known Concentrations of reducing sugar. (µg/ml)

Enter unknown sample's absorbance reading at 540 nm

The estimated concentration of the unknown sample is: µg/ml

Using eq (forced zero intercept): X = Y / m

Where
Y = : Unknown sample absorbance,
m = : Slope (b=0)