| Standard Samples Setup. |
| Sr. no |
Stock Soln. (ml) |
Dist. water (ml) |
Conc. of stock (µg/ml) |
DNS Reagent(ml) |
Incubation |
40% Rochelle Salt (ml) |
O.D 540nm |
| Blank |
0.0 |
3.0 |
0.0 |
3 |
Incubate at room temperature for 5 to 10 min |
1 |
0 |
| 1 |
0.5 |
2.5 |
100 |
0.22 |
| 2 |
1.0 |
2.0 |
200 |
0.40 |
| 3 |
1.5 |
1.5 |
300 |
0.61 |
| 4 |
2.0 |
1.0 |
400 |
0.82 |
| 5 |
2.5 |
0.5 |
500 |
1.2 |
| Test Sample Setup. |
| Sr. no |
Stock Soln. (ml) |
Dist. water (ml) |
Conc. of stock (µg/ml) |
DNS Reagent(ml) |
Incubation |
40% Rochelle Salt (ml) |
O.D 540nm |
| 1.0 |
2.0 |
2.5 |
? |
3 |
Incubate at room temperature for 5 to 10 min |
1 |
0.76 |