This calculator uses a standard curve plot to calculate the concentration of unknown samples. Check sample data table (Open Table)

Next Step
Enter absorbance reading at 660nm and known BSA Concentration (µg/ml)
Enter unknown sample's absorbance reading at 660nm

The estimated concentration of the unknown sample is: µg/ml

Using eq: X=(Y-b)/m
Where
Y=: Unknown sample absorbance,
b=: Intercept at Y
m=: Slope