| Standard Samples Setup. |
| Sr. no |
Stock solution (BSA) |
Conc. of stock (µg/ml) |
Distilled water (ml) |
Reagent C (ml) |
Incubation |
Folin Ciocalteu reagent (ml) |
Incubation |
O.D 660nm |
| 1 |
0.2 |
200 |
0.8 |
4 |
Incubate at room temperature for 10 min |
0.4 |
Incubate at room temperature for 15 min |
0.049 |
| 2 |
0.4 |
400 |
0.6 |
4 |
0.4 |
0.077 |
| 3 |
0.6 |
600 |
0.4 |
4 |
0.4 |
0.096 |
| 4 |
0.8 |
800 |
0.2 |
4 |
0.4 |
0.122 |
| 5 |
1.0 |
1000 |
0.0 |
4 |
0.4 |
0.151 |
| Test and Blank Sample Setup. |
| Sr. no |
Stock solution (BSA) |
Conc. of stock (µg/ml) |
Distilled water (ml) |
Reagent C (ml) |
Incubation |
Folin Ciocalteu reagent (ml) |
Incubation |
O.D 660nm |
| Blank |
0 |
0 |
1 |
4 |
Incubate at room temperature for 10 min |
0.4 |
Incubate at room temperature for 15 min |
0.00 |
| Test Sample |
0.4 |
? |
0.6 |
4 |
0.4 |
0.082 |