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Enzyme-linked immunosorbent assay (ELISA) using protein and antibody

1. What is the primary purpose of an ELISA test?

a: To detect the presence of antigens or antibodies in a sample Explanation

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b: To detect the presence of DNA in a sample Explanation

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c: To measure the pH of a sample Explanation

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d: To measure the conductivity of a sample Explanation

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2. What is the name of the ELISA variant that involves using two antibodies to detect an antigen?

a: Direct ELISA Explanation

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b: Indirect ELISA Explanation

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c: Sandwich ELISA Explanation

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d: Competitive ELISA Explanation

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3. In which type of ELISA test is a labeled antigen used instead of a labeled antibody?

a: Direct ELISA Explanation

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b: Sandwich ELISA Explanation

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c: Indirect ELISA Explanation

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d: Competitive ELISA Explanation

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4. Which of the following is not a type of ELISA?

a: Direct ELISA Explanation

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b: Sandwich ELISA Explanation

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c: Competitive ELISA Explanation

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d: Western blot ELISA Explanation

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5. Which of the following is a common substrate used in ELISA tests?

a: HRP Explanation

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b: BSA Explanation

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c: NaCl Explanation

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d: EDTA Explanation

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6. What type of substrate is used in ELISA to produce a colored product?

a: Fluorogenic substrate Explanation

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b: Chromogenic substrate Explanation

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c: Radiolabeled substrate Explanation

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d: Chemiluminescent substrate Explanation

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7. Which type of ELISA involves the direct detection of an antigen with an enzyme-labeled antibody?

a: Indirect ELISA Explanation

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b: Sandwich ELISA Explanation

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c: Direct ELISA Explanation

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d: Competitive ELISA Explanation

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8. What is the role of the enzyme-conjugated secondary antibody in an ELISA?

a: To bind directly to the antigen Explanation

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b: To facilitate the capture of the antigen Explanation

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c: To detect the antigen-antibody complex Explanation

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d: To inhibit the antigen Explanation

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9. What is the principle behind a competitive ELISA?

a: An enzyme-labeled antibody directly detects the antigen. Explanation

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b: The antigen is captured between two antibodies. Explanation

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c: The antigen competes with a labeled antigen for binding to an antibody. Explanation

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d: A primary antibody is followed by an enzyme-labeled secondary antibody. Explanation

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10. What does a colored product in an ELISA indicate?

a: The presence of a chromogenic substrate Explanation

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b: The presence of an antigen or antibody in the sample Explanation

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c: The inhibition of the enzymatic reaction Explanation

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d: The failure of the assay Explanation

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