Virtual Labs

DNA amplification by Polymerase Chain Reaction (PCR)

1. Which of the following statements is accurate for the PCR – polymerase chain reaction?

a: Automated PCR machines are called thermal cyclers Explanation

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b: A thermostable DNA polymerase is required Explanation

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c: Millions of desired DNA copies can be produced from microgram quantities of DNA Explanation

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d: All of the above Explanation

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2. Which of the following is an application of using Polymerase Chain Reaction?

a: DNA cloning Explanation

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b: Medical diagnostics Explanation

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c: Forensic analysis Explanation

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d: All of the above Explanation

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3. Primers used for PCR are

a: Single-stranded DNA oligonucleotide Explanation

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b: Double-stranded RNA oligonucleotide Explanation

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c: Double-stranded DNA oligonucleotide Explanation

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d: Single-stranded RNA oligonucleotide Explanation

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4. Taq polymerase used for DNA amplification in PCR is isolated from

a: Thermus Aquaticus Explanation

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b: Homo Sapiens Explanation

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c: Saccharomyces Cerevisiae Explanation

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d: Escherichia Coli Explanation

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5. Which of the following is not a component of the PCR reaction mixture?

a: dNTPs Explanation

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b: DNA ploymerase Explanation

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c: RNA polymerase Explanation

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d: Primers Explanation

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6. DNA polymerase used in PCR must have the following characteristics

a: Thermostability Explanation

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b: High fidelity Explanation

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c: High processivity Explanation

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d: All of the above Explanation

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7. How many types of dNTPs are required for PCR?

a: 1 Explanation

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b: 2 Explanation

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c: 3 Explanation

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d: 4 Explanation

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8. Optimum GC content for primer design for PCR must be in the range of

a: 40%- 60% Explanation

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b: 90%- 100% Explanation

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c: 20%- 30% Explanation

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d: 80%- 90% Explanation

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9. Polymerase chain reaction (PCR) was invented by

a: Kary Mullis Explanation

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b: Ulrich K. Laemmli Explanation

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c: Arne Tiselius Explanation

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d: Maurice Wilkins Explanation

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10. Which of the following is a thermostable polymerase?

a: Pfu polymerase Explanation

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b: Taq polymerase Explanation

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c: Both a and b Explanation

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d: DNA polymerase III Explanation

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