Electron Microscopy For Beginners

  1. Optimize the beam size in order to focus only on the region the diffraction pattern has to be obtained. Focus on a crystalline region.
  2. Obtain the diffraction pattern for a particular zone axis. The symmetry will pertain to the symmetry of real crystal.
  3. In order to obtain the 3-D symmetry information of the crystal, orient the crystal differently and obtain the diffraction pattern at other orientations as well.
  4. Take the print-out of the diffraction pattern (or utilize any other software) and obtain the physical parameters from the image.
  5. Obtain the camera constant and from the (hkl) plane, and calculate the lattice parameter of the crystal.
  6. Index the diffraction patterns using the similarity of crystal systems