Virtual Labs

Construction of de Bruijn Graphs for Sequence Assembly

1. The sequencing technology that benefits most from the De-Burgin graph-based sequence assembly is

a: Sanger sequencing Explanation

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b: Next-generation sequencing Explanation

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c: Third-Generation Sequencing Explanation

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d: PacBio Sequencing Explanation

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2. Sequence assembly refers to

a: Division of the large pieces of DNA into small pieces and reading the small fragments that constitute the original DNA molecule by connecting the information on various short fragments Explanation

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b: Segmenting the smaller pieces of DNA to study the original sequence of the given DNA molecule. Explanation

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c: Integrating the smaller pieces of DNA to larger DNA sequences to study the sequence assembly of the given DNA molecule. Explanation

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d: Division of the large pieces of DNA into small pieces of DNA for sequence alignment. Explanation

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3. Finding overlaps is specific for

a: De Bruijn Graph approach Explanation

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b: Fitch Margolish Algorithm Explanation

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c: Overlap-Layout-Consensus approach Explanation

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d: Viterbi algorithm Explanation

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4. Identify the major challenge in the construction of De Bruijn graphs.

a: Repetitive genomic regions Explanation

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b: Selection of k-mer Explanation

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c: Sequencing errors Explanation

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d: All the above Explanation

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5. Choose the primary purpose of constructing a De Bruijn graph in genome assembly.

a: Alignment of sequencing reads to a reference genome Explanation

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b: Counting the frequency of nucleotides in a genome Explanation

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c: Representation of k-mer overlaps for sequence reconstruction Explanation

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d: Finding the shortest path in a genome sequence Explanation

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