To Study the operational details of a CD spectrometer

1. Nitrogen purging should be done:
2. Reason for purging Nitrogen in the chamber of the CD spectrometer is:
3. Why it is essential to run a blank spectrum with buffer or solvent system before measuring the spectrum for sample:
4. Why the regulation of temperature of the cell holder is important:
5. How much time is required for the machine to warm up and achieve stability
6. The optimum path length generally required for: