The first step is the preparation of substrate solutions of different concentrations ( See Table ). For this, click on the rack of microcentrifuge tubes.

Next Step
28 28 28 28 Substrate of different concentration
(s6, s5, s4, s3, s2, s1)
Substrate Solution
(M. lysodeikticus).
Phosphate Buffer
(pH ~7.2)
Micro Pipettes