Step 1: Preparation of Microcarrier Particles
Select correct microcarrier material
Silver
Gold
Copper
Tungsten
Check
Step 2: DNA Coating on Microcarriers
Arrange the steps in correct order
(1) Mix DNA + CaCl
2
(2) Add microcarrier particles
(3) Incubate to allow DNA precipitation
(4) Wash and resuspend particles
Enter number in the below textboxes
->
->
->
Check
Step 3: : Loading into the Gene Gun
Identify correct component for loading
Handle
Pressure gauge
Macrocarrier holder
Check
Step 4: Pressure Activation
Choose correct helium pressure
100 psi
900 - 1100 psi
2000 psi
Step 5: Macrocarrier Stopping
Why is the stopping screen needed?
To cool the gas
To sterilize the chamber
To stop the macrocarrier before it hits tissue
Step 6: Distance Between Tissue and Screen
Enter distance:
Select the correct distance
3 cm
12 cm
6-9 cm
Step 7: Post-Bombardment Processing
Select the correct Post-Bombardment Processing
Wash thoroughly
Incubate on selective medium
Discard non-bombarded tissue
Alert Message