1. Upload Data File: Click "Choose file" to upload your input file (.txt/FASTA format)

2. Click "Sample Text file" to obtain a sample text file format or Click "Sample FASTA" to obtain a sample FASTA file format

3. Click to 'Run Algorithm' to get the result.

4. Click to 'Next Step' to do clustering.

6. Reset to start over

Step 1: Compute the Pairwise Distance Matrix

Step 2: Identify the Closest Pair of Taxa

Step 3: Create a new internal node that represents the common ancestor of the closest taxa.
▪Assign branch lengths to the new node using the weighted least-squares method.
▪The branch length from taxa to the new node is computed as: Where 𝑋 is the new node, and 𝑛 is the total number of taxa.

Step 4: Update the Distance Matrix ▪Compute distances from X to all other taxa using the formula:

Step 5: Repeat Steps 2-4 Until Only One Node Remains

Step 6: Construct the Phylogenetic Tree