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Standard Samples Setup
Test tubes	Vol of standard BSA (µl)	Vol of D/W (µl)	Conc. of BSA (µg/ml)	Vol. of Bradford’s reagent (ml)	OD at 595 nm
1	50	450	5	2.5 ml	0.023
2	100	400	10		0.035
3	150	350	15		0.042
4	200	300	20		0.042
5	250	250	25		0.052
6	300	200	30		0.057
7	350	150	35		0.053
8	400	100	40		0.068
9	450	50	45		0.081
10	500	0	50		0.104

Test and Blank Sample Setup
Test tubes	Vol of Protein sample (µl)	Vol of D/W (µl)	Conc. of BSA (µg/ml)	Vol. of Bradford’s reagent (ml)	OD at 595 nm
Blank	0	500	0	2.5 ml	0.000
Test Sample	100	400	?		0.038