Experimental Setup Tables.
Next Step| Standard Sample Setup Table | ||||||||
|---|---|---|---|---|---|---|---|---|
| Sr. no | BSA (ml) | Distilled water (ml) | BSA Concentration (µg/ml) | BCA Reagent (ml) | Incubation | Absorbance 562nm | ||
| 1 | 0.2 | 0.8 | 20 | 2ml | Incubate at room temperature for 30 min | 0.2 | ||
| 2 | 0.4 | 0.6 | 40 | 0.5 | ||||
| 3 | 0.6 | 0.4 | 60 | 0.8 | ||||
| 4 | 0.8 | 0.2 | 80 | 0.9 | ||||
| 5 | 1.0 | 0.0 | 100 | 1.2 | ||||
| Test and Blank Sample Table. | ||||||||
|---|---|---|---|---|---|---|---|---|
| Sr. no | BSA (ml) | Distilled water (ml) | BSA Concentration (µg/ml) | BCA Reagent (ml) | Incubation | Absorbance 562nm | ||
| Blank | 0 | 1 | 0 | 2ml | Incubate at room temperature for 30 min | 0.00 | ||
| Test Sample | 0.2 | 0.8 | ? | 1.1 | ||||