Experimental Setup Tables.

Next Step

Standard Sample Setup Table
Sr. no BSA (ml) Distilled water (ml) BSA Concentration (µg/ml) BCA Reagent (ml) Incubation Absorbance 562nm
1 0.2 0.8 20 2ml Incubate at room temperature for 30 min 0.2
2 0.4 0.6 40 0.5
3 0.6 0.4 60 0.8
4 0.8 0.2 80 0.9
5 1.0 0.0 100 1.2


Test and Blank Sample Table.
Sr. no BSA (ml) Distilled water (ml) BSA Concentration (µg/ml) BCA Reagent (ml) Incubation Absorbance 562nm
Blank 0 1 0 2ml Incubate at room temperature for 30 min 0.00
Test Sample 0.2 0.8 ? 1.1