To Study the effect of Secondary Structure elements on CD spectrum of proteins.
1) For each of the molecule coloured in blue(carbon atoms) you have to find chiral/asymmetric ones(ones with all four groups attached to it are pairwise different...
2) If the directly attached atoms are same for two or more groups go to the next attached to these attached atoms until you find some difference...
3) If atleast two of the attached atoms are same for any carbon atoms than it is for sure not asymmetric...
4) If you find out some carbon atom to be chiral than click it if it changes color than you are correct otherwise you are wrong
5) Click it again to revert the color back to blue
Experiment 5: Operational Details of a CD Spectrometer.